Developmental control of the heat-shock stress regulon in Streptomyces coelicolor |
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Authors: | Anna Maria Puglia Jiri Vohradsky Charles J. Thompson |
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Affiliation: | Universitádi Palermo, Dipartimento di Biologia Cellulare e dello Sviluppo, Sezione di Genetica, Parco d"Orleans, 90128 Palermo, Italy; Czech Academy of Sciences, Institute of Microbiology, Videnska 1083, CZ-14220 Praha-4, Czech Republic; Biozentrum, University of Basel, Department of Microbiology, Klingelbergstrasse 70, CH-4056 Basel, Switzerland; Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, France |
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Abstract: | In the differentiating eubacterium Streptomyces coelicolor , nutritional imbalances activate a developmental programme which involves the heat-shock stress regulon. In liquid batch cultures, the growth curve could be separated into four components: rapid growth 1 (RG1), transition (T), rapid growth 2 (RG2) and stationary (S). Patterns of gene expression in cultures subjected to heat shock in various phases were recorded on two-dimensional gels and analysed using advanced statistical methods. The responses of all heat-shock proteins (HSPs) were highly dependent upon the growth phase, thus demonstrating that the four phases of growth were physiologically distinct. For many HSPs, the levels of thermal induction attained were closely related to growth stage-determined levels of synthesis before heat shock, thus supporting the idea that developmental and thermal induction of this stress regulon have common control elements. Cluster analysis identified five groups of HSPs displaying similar kinetics of heat and developmentally induced synthesis, probably reflecting the influence of major regulatory systems. Methods introduced here to analyse the response of groups of genes to multiple simultaneous stimuli should find broad applications to studies of other prokaryotic and eukaryotic regulons. |
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