Mass characteristics of DNA obtained from chromosomes of a human carcinoma cell line |
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| Authors: | Shawn K Stickel Roger William Clark |
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| Institution: | (1) Department of Biological Sciences, University of Denver, 80208 University Park, Denver, CO, USA |
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| Abstract: | Sedimentation studies of DNA from chromosomes extracted from human mitotic cells showed that highmolecular-weight DNA can be obtained if cell hypotonic treatments and prolonged metaphase blocks are avoided. Two types of large double-stranded DNA were observed. One of these (M
r
= 2.5×108) appeared as a size class with characteristics reminiscent of the chromosomal DNA subunit hypothesis. However, this DNA is the decay product of larger molecules, whose minimum molecular weight is 6×108. |
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