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Assay of 2-Hydroxyputrescine in Various Regions of Rat Brain by Gas Chromatography-Mass Spectrometry
Authors:Tadashi Noto  Takeshi Hasegawa  Hiromichi Hashimoto  Teruo Nakajima
Affiliation:Department of Psychiatry, Kyoto Prefectural University of Medicine, Japan.
Abstract:2-Hydroxyputrescine in seven regions of single rat brains was measured with a sensitive, specific assay by gas chromatography-mass spectrometry. The regions were the cerebral cortex, cerebellum, medulla oblongata, hypothalamus, striatum, hippocampus, and midbrain. The level of 2-hydroxyputrescine was very high in the cerebral cortex and cerebellum, high in the medulla oblongata, hypothalamus, and hippocampus, and low in the striatum and midbrain. The level of 2-hydroxyputrescine in the cerebellum was significantly higher than in the striatum and midbrain.
Keywords:Regional distribution    Hydroxyputrescine    Polyamines    Brain    Gas chromatography-mass spectrometry.
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