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Environmental Isolates of Aeromonas spp. Harboring the cagA-Like Gene of Helicobacter pylori
Authors:Simanti Datta  Asis Khan  Ranjan K Nandy  Motiur Rehman  Sutapa Sinha  Santanu Chattopadhyay  Suresh C Das  and G Balakrish Nair
Abstract:We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.
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