| PARP10组织分布、相互作用蛋白及UV应激反应的分析 |
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| 引用本文： | 于孟斌,赵莉霞,杨予涛,杨志新,张莹莹,朱恒奇,周晓巍,黄培堂. PARP10组织分布、相互作用蛋白及UV应激反应的分析[J]. 生物工程学报, 2009, 25(3): 428-434 |
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| 作者姓名： | 于孟斌 赵莉霞 杨予涛 杨志新 张莹莹 朱恒奇 周晓巍 黄培堂 |
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| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100071;防化指挥工程学院生物防护教研室,北京,102205
2. 军事医学科学院生物工程研究所,北京,100071 |
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| 基金项目： | 国家科技支撑计划(No.2006BAD06A01)资助~~ |
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| 摘 要： | 根据GenBank中公布的人多聚腺苷酸二磷酸核糖聚合酶10(PARP10)cDNA序列,设计并合成一对特异性引物,通过RT-PCR扩增293FT细胞mRNA,获得PARP10cDNA。将获得的cDNA克隆到pCMV-Myc和pEGFP-C1中,用免疫沉淀和激光共聚焦实验验证了PARP10和β-actin存在相互作用。然后,通过RT-PCR法发现该基因在小鼠体内表达的组织分布,组织表达谱显示该蛋白在各组织中均有表达;Westernblotting分析表明,UV造成的细胞损伤能够引起PARP10表达水平的增高。PARP10组织表达谱的确定,与β-actin相互作用的验证以及对UV的应激反应都为进一步研究PARP10的生物学功能奠定了基础。
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| 关 键 词： | PARP10 UV 组织表达谱 免疫沉淀 激光共聚焦 |
| 收稿时间： | 2008-10-06 |
Analysis of PARP10 tissue expression profile, interactive protein and UV stress reaction |
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| Mengbin Yu,Lixia Zhao,Yutao Yang,Zhixin Yang,Yingying Zhang,Hengqi Zhu,Xiaowei Zhou and Peitang Huang. Analysis of PARP10 tissue expression profile, interactive protein and UV stress reaction[J]. Chinese journal of biotechnology, 2009, 25(3): 428-434 |
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| Authors: | Mengbin Yu Lixia Zhao Yutao Yang Zhixin Yang Yingying Zhang Hengqi Zhu Xiaowei Zhou Peitang Huang |
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| Affiliation: | Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China; Laboratory of Biology Defense, Command and Engineering College of Chemical Defense, Beijing 102205, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China;Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China |
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| Abstract: | |
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| Keywords: | PARP10 ultraviolet ray tissue expression profile immuno-precipitation laser confocal microscopy |
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