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| 质粒pAAV-HRE-CD151的构建和鉴定 | |
| 引用本文： | 魏全,林敬阳,左后娟,费宇杰,彭丹,黄晓琳,刘正湘.质粒pAAV-HRE-CD151的构建和鉴定[J].中国组织化学与细胞化学杂志,2011,20(2):112-115. |
| 作者姓名： | 魏全 林敬阳 左后娟 费宇杰 彭丹 黄晓琳 刘正湘 |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院康复科,武汉,430030 2. 浙江省人民医院心内科,杭州,310014 3. 华中科技大学同济医学院附属同济医院心内科,武汉,430030 |
| 基金项目： | 国家自然科学基金资助(30670856,81000047) |
| 摘 要： | 目的为了增加CD151基因转染大鼠缺血心肌的效率和靶向特异性。方法以质粒pAAV-CD151为模板,采用克隆技术,构建一个含缺氧反应元件(hypoxia response element,HRE)和CD151基因序列的腺相关病毒载体,通过HRE促进CD151在大鼠缺血心肌的表达。结果经过测序鉴定成功构建了含缺氧反应元件的质粒pAAV-HRE-CD151。结论成功构建了pAAV-HRE-CD151质粒,为CD151治疗缺血性心血管病的靶向研究奠定了基础。 |
| 关 键 词： | 心肌缺血 CD151 HRE 基因治疗 pAAV-HRE-CD151 |
Construction and Identification of the plasmid pAAV-HRE-CD151 |
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| Wei Quan,Lin Jingyang,Zuo Houjuan,Fei Yujie,Peng Dan,Huang Xiaolin,Liu Zhengxiang.Construction and Identification of the plasmid pAAV-HRE-CD151[J].Chinese Journal of Histochemistry and Cytochemistry,2011,20(2):112-115. | |
| Authors: | Wei Quan Lin Jingyang Zuo Houjuan Fei Yujie Peng Dan Huang Xiaolin Liu Zhengxiang |
| Institution: | Wei Quan1,Lin Jingyang2,Zuo Houjuan3,Fei Yujie3,Peng Dan3,Huang Xiaolin1,Liu Zhengxiang3 (1Department of Rehabilitation Medicine,Tong ji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,2Department of Cardiology of Zhejiang Provincial People's Hospital,Hangzhou 310014,3Department of cardiology,Tongji Hospital,Tongji Medical college,China) |
| Abstract: | Objective To improve the delivery efficacy and target specificity of the pro-angiogenic gene CD151 to the ischemic heart Methods Taking pAAV-CD151 plasmid as the template and using clone technology,an AAV construct which includes hypoxia response element(HRE) and CD151 gene was generated to improve CD151 gene expression in the rat ischemic myocardiumResults Plasmid pAAV-HRE-CD151 was successfully constructed,and the result was confirmed by sequencingConclusion Plasmid pAAV-HRE-CD151 was successfully constru... |
| Keywords: | Cardiac ischemia CD151 HRE Gene therapy pAAV-HRE-CD151 |
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