Identification of the Protein Target of Myelin-Binding Ligands by
Immunohistochemistry and Biochemical Analyses |
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| Authors: | Anshika Bajaj Nicole E LaPlante Victoria E Cotero Kenneth M Fish Roger M Bjerke Tiberiu Siclovan Cristina A Tan Hehir |
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| Institution: | GE Global Research, One Research Circle, Niskayuna, New York (AB,NEL,VEC,KMF,TS,CATH);GE Healthcare, Varemottak, Oslo, Norway (RMB) |
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| Abstract: | The ability to visualize myelin is important in the diagnosis of demyelinating disorders
and the detection of myelin-containing nerves during surgery. The development of
myelin-selective imaging agents requires that a defined target for these agents be
identified and that a robust assay against the target be developed to allow for assessment
of structure-activity relationships. We describe an immunohistochemical analysis and a
fluorescence polarization binding assay using purified myelin basic protein (MBP) that
provides quantitative evidence that MBP is the molecular binding partner of previously
described myelin-selective fluorescent dyes such as BMB, GE3082, and GE3111. |
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| Keywords: | fluorescence polarization myelin basic protein nerves myelin dye |
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