Pathogen Induced Changes in the Protein Profile of Human Tears from Fusarium Keratitis Patients |
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Authors: | Sivagnanam Ananthi Namperumalsamy Venkatesh Prajna Prajna Lalitha Murugesan Valarnila Kuppamuthu Dharmalingam |
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Institution: | 1. Dr. G. Venkataswamy Eye Research Institute, Aravind Medical Research Foundation, Aravind Eye Care System, Madurai, India.; 2. Cornea Clinic, Aravind Eye Hospital, Aravind Eye Care System, Madurai, India.; 3. Department of Microbiology, Aravind Eye Hospital, Aravind Eye Care System, Madurai, India.; 4. School of Biotechnology, Madurai Kamaraj University, Madurai, India.; New York State Health Department and University at Albany, United States of America, |
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Abstract: | Fusarium is the major causative agent of fungal infections leading to corneal ulcer (keratitis) in Southern India and other tropical countries. Keratitis caused by Fusarium is a difficult disease to treat unless antifungal therapy is initiated during the early stages of infection. In this study tear proteins were prepared from keratitis patients classified based on the duration of infection. Among the patients recruited, early infection (n = 35), intermediate (n = 20), late (n = 11), samples from five patients in each group were pooled for analysis. Control samples were a pool of samples from 20 patients. Proteins were separated on difference gel electrophoresis (DIGE) and the differentially expressed proteins were quantified using DeCyder software analysis. The following differentially expressed proteins namely alpha-1-antitrypsin, haptoglobin α2 chain, zinc-alpha-2-glycoprotein, apolipoprotein, albumin, haptoglobin precursor - β chain, lactoferrin, lacrimal lipocalin precursor, cystatin SA III precursor, lacritin precursor were identified using mass spectrometry. Variation in the expression level of some of the proteins was confirmed using western blot analysis. This is the first report to show stage specific tear protein profile in fungal keratitis patients. Validation of this data using a much larger sample set could lead to clinical application of these findings. |
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