Identification of a Residue Affecting Fatty Alcohol Selectivity in Wax Ester Synthase |
| |
| Authors: | Brett M Barney Rachel L Mann Janet M Ohlert |
| |
| Institution: | Department of Bioproducts and Biosystems Engineering and BioTechnology Institute, University of Minnesota, St. Paul, Minnesota, USA |
| |
| Abstract: | The terminal enzyme in the bacterial wax ester biosynthetic pathway is the bifunctional wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT), which utilizes a fatty alcohol and a fatty acyl-coenzyme A (CoA) to synthesize the corresponding wax ester. In this report, we identify a specific residue in WS/DGAT enzymes obtained from Marinobacter aquaeolei VT8 and Acinetobacter baylyi that alters fatty alcohol selectivity and kinetic parameters when modified to alternative residues. |
| |
| Keywords: | |
|
|
