Use of Flow Cytometry for Rapid,Quantitative Detection of Poliovirus-Infected Cells via TAT Peptide-Delivered Molecular Beacons |
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| Authors: | Divya Sivaraman Hsiao-Yun Yeh Ashok Mulchandani Marylynn V. Yates Wilfred Chen |
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| Affiliation: | aDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA;bDepartment of Chemical and Environmental Engineering, University of California, Riverside, California, USA;cDepartment of Environmental Sciences, University of California, Riverside, California, USA |
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| Abstract: | Rapid and efficient detection of viral infection is crucial for the prevention of disease spread during an outbreak and for timely clinical management. In this paper, the utility of Tat peptide-modified molecular beacons (MBs) as a rapid diagnostic tool for the detection of virus-infected cells was demonstrated. The rapid intracellular delivery mediated by the Tat peptide enabled the detection of infected cells within 30 s, reaching saturation in signal in 30 min. This rapid detection scheme was coupled with flow cytometry (FC), resulting in an automated, high-throughput method for the identification of virus-infected cells. Because of the 2-order-of-magnitude difference in fluorescence intensity between infected and uninfected cells, as few as 1% infected cells could be detected. Because of its speed and sensitivity, this approach may be adapted for the practical diagnosis of multiple viral infections. |
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