Direct Observation of Single DNA Structural Alterations at Low Forces with Surface-Enhanced Raman Scattering |
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| Authors: | Satish Rao Saurabh Raj Benjamin Cossins Monica Marro Victor Guallar Dmitri Petrov |
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| Institution: | †ICFO - Institut de Ciències Fotòniques, Castelldefels (Barcelona), Spain;‡Joint BSC-IRB Research Program in Computational Biology, Barcelona Supercomputing Center, Barcelona, Spain;§ICREA - Institucio Catalana de Recerca i Estudis Avancats, Barcelona, Spain |
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| Abstract: | DNA experiences numerous mechanical events, necessitating single-molecule force spectroscopy techniques to provide insight into DNA mechanics as a whole system. Inherent Brownian motion limits current force spectroscopy methods from observing possible bond level structural changes. We combine optical trapping and surface-enhanced Raman scattering to establish a direct relationship between DNA’s extension and structure in the low force, entropic regime. A DNA molecule is trapped close to a surface-enhanced Raman scattering substrate to facilitate a detectable Raman signal. DNA Raman modes shift in response to applied force, indicating phosphodiester mechanical alterations. Molecular dynamic simulations confirm the local structural alterations and the Raman sensitive band identified experimentally. The combined Raman and force spectroscopy technique, to our knowledge, is a novel methodology that can be generalized to all single-molecule studies. |
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