Yeast gene which suppresses the defect in protein export of a secY mutant of E. coli |
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Authors: | M Sakaguchi C Ueguchi K Ito T Omura |
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Institution: | Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka. |
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Abstract: | To find factors participating in protein translocation in yeast, we screened a yeast genomic library for genes which, when introduced into Escherichia coli, suppressed secY24, a temperature sensitive mutation of an essential integral membrane protein (SecY) required for protein export. We isolated and characterized a gene (YSY6) which improved the translocation of the OmpA protein in mutant strain IQ85(secY24). It could also suppress another mutant rplO215(Am)], in which the level of expression of the SecY protein is decreased at high-temperature. The YSY6 gene encodes a small amphiphilic peptide consisting of 65 amino acids, which can be expressed in E. coli cells. |
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