Polyamine-stimulated phosphorylation of proteins from corn (Zea mays L.) coleoptiles |
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| Authors: | K Veluthambi B W Poovaiah |
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| Institution: | Department of Horticulture and Landscape Architecture Washington State University Pullman, Washington 99164-6414 USA |
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| Abstract: | The effect of polyamines (spermine, spermidine and putrescine) on in vitro phosphorylation of proteins from corn coleoptiles was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Spermine promoted the phosphorylation of several membrane and soluble proteins and most of the proteins phosphorylated were different from those phosphorylated in the presence of calcium. Spermidine promoted the phosphorylation to a lesser extent and putrescine had very little stimulatory effect. Spermine-promoted phosphorylation of soluble proteins was dependent upon the presence of Mg2+ and was discernible at 100 microM spermine concentration. |
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| Keywords: | EGTA ethylene glycol-bis (β-amino-ethyl ether) N N N′ N′-tetraacetic acid Mes 2-[N-morpholino]-ethanesulfonic acid DTT dithiothreitol |
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