IN VITRO EFFECT OF SUCROSE FEEDING IN THE DIFFERENTIATION OF WOOD ELEMENTS OF PLUMERIA |
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Authors: | LILY RAY SRILEKHA GHOSH P. C. DATTA |
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Affiliation: | Botanical Lab. of Pharmaco-anatomy, University of Calcutta, Calcutta-700019, India |
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Abstract: | Wood with cambium of Plumeria rubra Linn. was cultured in modified Schenk and Hildebrandt medium for 75 days. Change in dimension and frequency of cells was compared with that grown in situ for 75 days. Feeding of 41% sucrose in vitro brought about the same type of differentiation as occurred in situ , where non-reducing sugar percentage increased (from 0.0004 before culture to 0.0032 after 75 days' growth in situ ). Sucrose favoured enlargement of axial elements and numerical increase of all lignified elements. |
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