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Effects of Pluronic F-68 on plant cells in suspension culture
Authors:A T King  M R Davey  B J Mulligan  K C Lowe
Institution:(1) Departments of Zoology and Botany, University of Nottingham, University Park, NG7 2RD Nottingham, UK
Abstract:Summary The effects of the non-ionic surfactant, Pluronic F-68, on growth and structure ofSolanum dulcamara cells in suspension culture have been studied. Growth of cells, as measured by dry weight, was unaffected by low concentrations (0.01–1.0% w/v) of pluronic, while culture with higher concentrations (2.5–10.0%) resulted in cell death. It is suggested that low concentrations of pluronic may be valuable supplements in plant cell cultures to protect against mechanical damage and to manipulate membrane systems.
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