首页 | 本学科首页   官方微博 | 高级检索  
     


Localization of ornithine decarboxylase in the chick embryo during organogenesis
Authors:Bertil Löwkvist  Hadar Emanuelsson  Lo Persson  Frank Sundler  Anders Lundquist  Dr. Olle Heby
Affiliation:(1) Department of Zoophysiology, University of Lund, Lund, Sweden;(2) Department Physiology and Biophysics, University of Lund, Lund, Sweden;(3) Department Histology, University of Lund, Lund, Sweden;(4) Department of Zoophysiology, University of Lund, Helgonavägen 3B, pcS-223, 62 Lund, Sweden
Abstract:Summary The localization of ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis and thus in cell growth, was determined in the 4.5-day-old chick embryo, using two independent methods of analysis. ODC protein was identified by indirect immunofluorescence with a monospecific ODC antibody, and catalytically active ODC was identified by autoradiography with agr-(5-3H) difluoromethylornithine. Both methods revealed a basically similar distribution of ODC within the embryo. Among the organs, the brain exhibited the highest ODC levels. ODC levels were also high in spinal cord, mesonephric tubules and heart. Similar levels, but confined to limited areas, were found in liver tissue, head mesenchyme, and the oral and pharyngeal regions. Organs that exhibited high ODC levels are all engaged in rapid growth, as well as in extensive tissue remodeling and differentiation.
Keywords:Ornithine decarboxylase  Chick embryo  Organogenesis A  Autoradiography  Immunocytochemistry
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号