Binding of nicotinic ligands to and nicotine-induced calcium signaling in Trypanosoma cruzi |
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Authors: | Bollo M Venera G de Jiménez Bonino M B Machado-Domenech E |
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Institution: | Química Biológica, Facultad de Ciencias Exactas, Fisico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina. |
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Abstract: | This work provides evidence that nicotine (1 x 10(-5) M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by alpha-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC(50): 7.6 x 10(-7) M) and nicotine (IC(50): 1 x 10(-7) M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite. |
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