Rice gene expression in response to N-acetylchitooligosaccharide elicitor: comprehensive analysis by DNA microarray with randomly selected ESTs |
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| Authors: | Akimoto-Tomiyama Chiharu Sakata Katsumi Yazaki Junshi Nakamura Keiko Fujii Fumiko Shimbo Kanako Yamamoto Kimiko Sasaki Takuji Kishimoto Naoki Kikuchi Shoshi Shibuya Naoto Minami Eiichi |
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| Affiliation: | (1) Department of Biochemistry, Japan;(2) Department of Genome Research, Japan;(3) Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan;(4) STAFF Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, 305-0854, Japan |
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| Abstract: | N-acetylchitooligosaccharides are potent elicitors to suspension-cultured rice cells, inducing a set of defense reactions. Expression of defense-related genes is considered to play an important role in defense reactions, and we employed microarray analysis of 8987 randomly selected expressed sequence tags to analyze the changes in gene expression caused by N-acetylchitooctaose. In this experiment, 166 genes were significantly induced and 93 genes were repressed. RNA gel blot analysis of 16 of these genes confirmed the microarray results. Of the 259 ESTs identified as responsive to N-acetylchytooctaose, 18 genes are related to signal transduction, including five calcium-dependent protein kinases (CDPKs). Among these, three novel CDPKs responsive to N-acetylchitooctaose were isolated. |
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| Keywords: | CDPK microarray N-acetylchitooligosaccharides Oryza sativa rice cells |
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