大豆异黄酮对围绝经期大鼠卵巢Bax mRNA表达和Ca2+-ATP酶活性的影响

目的：观察大豆异黄酮（SI）对围绝经期大鼠卵巢BaxmRNA表达和Ca^2＋-ATP酶活性的影响。方法：采用自然老化法建立围绝经期大鼠动物模型。12月龄的初老雌性Wistar大鼠,分别给予低（50mg／kg）、中（158mg／kg）、高（500mg／kg）剂量SI灌胃处理8周。采用RT—PCR检测卵巢BaxmRNA的表达;采用化学比色法检测卵巢Ca^2＋-ATP酶活性、血清丙二醛（MDA）含量和超氧化物岐化酶（SOD）活性。结果：SI处理可使初老大鼠卵巢BaxmRNA表达和血清MDA含量降低,卵巢Ca^2＋-ATP酶活性和血清SOD活性明显升高（P〈0．05或P〈0、01）。结论：大豆异黄酮下调衰老卵巢BaxmRNA表达,提高细胞Ca2^2＋-ATP酶活性,可能是改善围绝经期卵巢功能的机制之一。

Effects of soy isoflavones on the expression of Bax mRNA and Ca(2+)-ATPase activity in ovaries of perimenopause rats

Aim: To investigate the effcts of soy isoflavones(SI) on the expression of Bax mRNA and Ca2+-ATPase activity in ovaries of perimenopause rats.Methods: The animal model of perimenopause rats was established by unforced aging.12 month-old praesenilis female Wistar rats were administered by intragastric(ig) with low(500 mg/kg),middle(158 mg/kg) and high(500 mg/kg) does of SI for 8 weeks.The expression of Bax mRNA in ovaries were detected by RT-PCR.Ca2+-ATPase activity in ovaries and MDA content and SOD activity in serum were detected by chemi-chromatometry.Results: Intervention of SI could significantly decrease the expression of Bax mRNA in ovaries and MDA content in serum,increase Ca2+-ATPase activity in ovaries and SOD activity in serum of praesenilis rats(P<0.05 or P<0.01).Conclusion: Soyisoflavones could down-regulate the expression of Bax mRNA and increase Ca2+-ATPase activity in aged ovaries.It is probably one of the mechanisms to improve the function of aged ovaries in perimenopause rats.