Efficacy of commercial products in enhancing oil biodegradation in closed laboratory reactors |
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Authors: | Albert D Venosa John R Haines Wipawan Nisamaneepong Rakesh Govind Salil Pradhan Belal Siddique |
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Institution: | (1) Risk Reduction Engineering Laboratory, U.S. Environmental Protection Agency, 45268 Cincinnati, OH, USA;(2) Technology Applications, Inc., Cincinnati, Ohio, USA;(3) University of Cincinnati, Cincinnati, Ohio, USA |
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Abstract: | Summary A laboratory screening protocol was designed and conducted to test the efficacy of eight commercial bacterial cultures and two non-bacterial products in enhancing the biodegradation of weathered Alaska North Slope crude oil in closed flasks. Three lines of evidence were used to support the decision to progress to field testing in Prince William Sound: rapid onset and high rate of oxygen uptake, substantial growth of oil degraders, and significant degradation of the aliphatic and aromatic hydrocarbon fractions of the weathered Alaska North Slope crude oil. A product had to enhance biodegradation greater than that achieved with excess mineral nutrients. Experiments were conducted in closed respirometer flasks and shake flasks, using seawater from Prince William Sound and weathered crude oil from a contaminated beach. Analysis of the data resulted in selection of two of the ten products for field testing. Both were bacterial products. Findings suggested that the indigenous Alaskan microorganisms were primarily responsible for the biodegradation in the closed flasks and respirometer vessels. |
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Keywords: | Bioaugmentation Hydrocarbon biodegradation Alkanes Aromatics Respirometry Oxygen uptake Crude oil |
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