Continuous cell lines from the common white, Pieris rapae crucivora boisduval |
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Authors: | Jun Mitsuhash Shouji Hayasaka Shigeo Imanishi |
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Institution: | (1) National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki Prefecture, Japan;(2) Department of Biosciences, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, 156-8502 Tokyo, Japan |
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Abstract: | Summary Three continuous cell lines, NIAS-PRC-819A, NIAS-PRC-819B, and NIAS-PRC-819C, were established from the pupal ovaries of the
common white, Pieris rapae crucivora Boisduval (Insecta, Lepidoptera, Pieridae). The primary culture was initiated as explant cultures with ovariole fragments
in MGM-464 medium supplemented with 20% fetal bovine serum at 25° C. About 6 mo after the culture was set up, the first subculture
was prepared. Thereafter, cells were subcultured with decreasing passage intervals, resulting in a cell population that multiplied
continuously. The karyotypes of these cell lines were similar to each other, and the majority of the cells showed about 100
microchromosomes. The population-doubling times of these cell lines were 3 to 7 d. The cell lines were susceptible to a microsporidia,
Nosema bombycis. Immunodiffusion experiments proved that these cell lines derived from the common white and not from other cell lines by
contamination. |
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Keywords: | cell culture insects lepidoptera ovaries |
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