Enhanced expression of a chromatin associated protein tyrosine phosphatase during G0 to S transition |
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Authors: | Sundaram Nambirajan R Sreek Antha Reddy Ghanshyam Swarup |
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Institution: | (1) Center for Cellular and Molecular Biology, Uppal Road, 500 007 Hyderabad, India |
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Abstract: | The non-transmembrane protein tyrosine phosphatase, PTP-S, is located predominantly in the cell nucleus in association with
chromatin. Here we have analysed the expression of PTP-S upon mitogenic stimulation and during cell division cycle. During
liver regeneration after partial hepatectomy, PTP-S mRNA levels increased 16-fold after 6 h (G1 phase) and declined thereafter. Upon stimulation of serum starved cells in culture with serum, PTP-S mRNA levels increased
reaching a maximum during late G1 phase and declined thereafter. No significant change in PTP-S RNA levels was observed in growing cells during cell cycle.
PTP-S protein levels were also found to increase upon mitogenic stimulation. Upon serum starvation for 72 h, PTP-S protein disappears
from the nucleus and is seen in the cytoplasm; after 96 h of serum starvation the PTP-S protein disappears from the nucleus
as well as cytoplasm. Refeeding of starved cells for 6 h results in reappearance of this protein in the nucleus. Our results
suggest a role of this phosphatase during cell proliferation. |
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Keywords: | Protein tyrosine phosphatase nuclear phosphatase liver regeneration cell cycle cell proliferation |
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