Ca2+/calmodulin-dependent protein kinase in Saccharomyces cerevisiae |
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Institution: | Research Laboratories of the Finnish State Alcohol Company (Alko), Box 350, SF-00101 Helsinki 10, Finland |
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Abstract: | Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 μM beef calmodulin the phosphorylation was optimal at 3 μM free Ca2+, and at 80 μM free Ca2+ it was half-maximal at about 0.1 μM beef calmodulin. It is concluded that the fraction contains calmodulin-dependent protein kinase(s) which is (are) autophosphorylated or associated with substrates. |
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