首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Flow cytometric analysis of chromosomes and cells using a modified BrdU-hoechst method
Authors:E Severin  B Ohnemus
Institution:(1) Department of Neurology, School of Medicine, Boston University, 80 East Concord Street, 02118 Boston, MA, USA;(2) Present address: Division of Neurology, Veterans Administration Medical Center, 150 S. Huntington Avenue, 02130 Boston, MA, USA
Abstract:Summary The sensory innervation of 46 poles of long chain intrafusal muscle fibers was studied histochemically by staining for NADH-TR in periodic, 8 mgrm thick transverse sections of cat muscle spindles. Each long chain fiber carried terminals of the primary sensory axon, and 23 of the fiber poles also displayed secondary sensory endings. With the NADH-TR reaction there was no apparent difference in the cross-sectional appearance of sensory endings on the long chain and on other nuclear chain fibers. However, the contact area between the secondary endings and the muscle fiber tended to be shorter on the long chain than on the neighboring chain fibers of shorter polar length. This was also the case for one long chain fiber in which the sensory innervation was examined in serial, 1 mgrm thick sections stained with toluidine blue. Discharges of the secondary sensory axons in cat spindles may be affected more by contraction of the shorter nuclear chain fibers than by activation of the long chain fibers.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号