血管活性肠多肽结合核苷酸性质的研究

采用光亲和技术检测了血管活性肠多肽(VIP)与核苷酸之间的相互作用,发现VIP可以特异性结合同位素标记的GTP,还发现未标记的GTP以及其它三磷酸核苷抑制这种结合,这意味着VIP与上述三磷酸核苷之间的结合是一种典型的可逆性的结合反应.发现低浓度的GDP,GMP不但不抑制VIP与同位素标记GTP的结合反应,反而增强这种结合.联系到其它研究者关于GTP影响VIP与其受体结合反应的结果,可认为VIP能可逆性地连接一种三磷酸核苷,这种连接受反应系统中不同核苷酸比例影响,通过这种连接来调节VIP与其受体之间的反应.

The Research on Vasoactive Intestinal peptide Binding With Nucleotides

The interaction between VIP and nucleotide was tested with advanced photo-affinity technique.It was found that VIP can bind radiolabled GTP specifically and this binding could be competitively inhibited by cold GTP(unlabled GTP).The experiment indicated that not only GTP could inhibit the binding between VIP and hot GTP, but also all other nucleoside triphophates such as ATP,TTP,UTP could competitively inhibit this binding,although their inhibitions were a little weaker than GTP.It means VIP binding nucleoside triphbsphate was a typical reversible binding reaction. It was found also that GDP, GMP at low concentration did not inhibit VIP binding hot GTP but enhanced the binding. Connecting with other researchers' results of GTP influencing on VIP-receptor interaction, it was considered that VIP could reversibly bind one of nucleoside triphosphates and this binding was modulated by the ratio of different nucleotides in reaction system. Through this binding, the interaction of VIP and it's receptor was regulated.