Chromosome transfer and Hfr formation by F in rec+ and recA strains of Escherichia coli K12. |
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Authors: | J Cullum P Broda |
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Institution: | Department of Molecular Biology, University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland |
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Abstract: | We attempted to assess the role of Hfr clones in chromosome transfer by F+ populations. We thought that any Hfr-independent component of fertility might be affected to a different extent by the recA mutation than was the Hfr component. However, the rate of Hfr formation and the efficiency of chromosome transfer were reduced to an equal extent (× 100-fold) by the recA mutation. Such experiments therefore provide no evidence for an Hfr-independent component. It appeared that Type II strains, which were thought to suffer a defect in Hfr formation, actually produced fertile clones but had a secondary defect which affected the persistence of these clones. Thus, evidence from Type II strains is also not useful for examining the quantitative contribution of Hfr cells to F+ transfer. |
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