Detection of 5S rDNA and other repeated DNA on supernumerary B chromosomes ofTriticum species (Poaceae) |
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Authors: | B Friebe J Jiang B Gill |
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Institution: | (1) Wheat Genetics Resource Center, Department of Plant Pathology, Throckmorton Hall, Kansas State University, 66506-5502 Manhattan, KS, USA |
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Abstract: | The supernumerary B chromosomes ofTriticum speltoides andT. tripsacoides were analyzed in mitotic metaphases of spike primordium cells by C-banding and in situ hybridization (ISH) analyzes. B chromosomes ofT. speltoides have larger telomeric and interstitial C-bands, whereas those ofT. tripsacoides are almost completely devoid of C-bands. A prominent ISH site of rye related DNA sequences (using probe pSc119) was detected on B chromosomes ofT. tripsacoides and only a minor ISH site was observed on theT. speltoides B chromosomes. However, two ISH sites of 5S rRNA loci were detected at a terminal and an interstitial location of theT. speltoides B chromosomes. These sites were absent on B chromosomes ofT. tripsacoides. The results are discussed with respect to the phylogenetic origin of these B chromosomes. |
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Keywords: | Poaceae Triticum speltoides T tripsacoides Supernumerary B chromosomes C-banding in situ hybridization |
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