Anaerobic respiration of Shewanella putrefaciens requires both chromosomal and plasmid-borne genes |
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| Authors: | D.A. Saffarini T.J. DiChristina D. Bermudes K.H. Nealson |
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| Affiliation: | Center for Great Lakes Studies, University of Wisconsin-Milwaukee, 600 East Greenfield Avenue, Milwaukee, WI 53204, USA; Biology Department, Woods Hole Oceanographic Institute, Woods Hole, MA, USA; Yale University, School of Medicine, Infectious Diseases Section, New Haven, CT, USA |
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| Abstract: | Abstract Pleiotropic respiratory mutants, incapable of growth on any electron acceptor other than oxygen, were isolated from two strains of Shewanella putrefaciens (MR-1 and sp200). All anaerobic respiratory functions were restored by complementation of the mutants with specific cloned DNA fragments. Southern hybridization experiments revealed that the fragment that complements the MR-1 mutant was localized on the megaplasmids of both strains, while the fragment that complements the sp200 mutant was chromosomal. Neither of these fragments hybridized with the anaerobic regulatory genes of S. putrefaciens ( etrA ) or E. coli ( fnr ). |
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| Keywords: | Anaerobic respiration Shewanella putrefaciens Respiratory mutant Iron reduction Manganese reduction |
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