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Fourth-derivative spectrophotometry of proteins
Authors:Esteve Padr  s, Mireia Du  ach, Antoni Morros, Manuel Sab  s,Joan Ma  osa
Affiliation:

The authors are at the Departament de Biofísica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra (Barcelona, Spain

Abstract:Fourth-derivative spectrophotometry offers several advantages over classical absorption or difference spectrophotometry in examining the characteristics of aromatic amino acids in proteins. The basic principles of the technique and its applications are outlined.
Keywords:
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