Fourth-derivative spectrophotometry of proteins |
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Authors: | Esteve Padr s, Mireia Du ach, Antoni Morros, Manuel Sab s,Joan Ma osa |
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Affiliation: | The authors are at the Departament de Biofísica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra (Barcelona, Spain |
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Abstract: | Fourth-derivative spectrophotometry offers several advantages over classical absorption or difference spectrophotometry in examining the characteristics of aromatic amino acids in proteins. The basic principles of the technique and its applications are outlined. |
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