Localization of C4 genes within the HLA complex by molecular genotyping |
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| Authors: | M. A. Robinson M. C. Carroll A. H. Johnson R. J. Hartzman K. T. Belt T. J. Kindt |
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| Affiliation: | (1) Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20205 Bethesda, Maryland;(2) Department of Biochemistry, MRC Immunochemistry Unit, Oxford University, Oxford, England;(3) Division of Immunologic Oncology, Lombardi Cancer Center, Georgetown University School of Medicine, Washington, DC;(4) Naval Medical Research Institute, 20205 Bethesda, Maryland |
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| Abstract: | Segregation of the complement component, C4, was analyzed in six families that each included an individual who inherited an HLA haplotype where a crossover event had occurred in the region between HLA-B and HLA-DR. Two cDNA clones corresponding to the C4 gene were utilized as probes in Southern blot analysis of DNA from members of each family. Restriction fragment length polymorphisms (RFLP) were observed and were assigned to haplotypes. In one family RFLP, hybridizing with the C4 probes, segregrated with HLA-B, and in four families RFLP segregated with HLA-DR; one family was not informative in this respect. These analyses have made it possible to localize the genes for C4 between HLA-B and HLA-DR by molecular genotyping and to characterize three different genomic configurations of C4 genes by limited restriction mapping.Abbreviations RFLP restriction fragment length polymorphisms - LCL lymphoblastoid cell lines |
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