The multi-locus H-2D
w16 region has an organization distinct from the D
d region |
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Authors: | Usha Tummuru M Kabir Zubairu David R Lee |
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Institution: | (1) Department of Molecular Microbiology and Immunology, M642 Medical Science Building, University of Missouri School of Medicine, 65212 Columbia, MO, USA |
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Abstract: | Genomic DNA blot analyses using probes derived from the BALB/c 3 flanking region of the L
d gene (L
d 3 fl-C) and from near the BALB/c D3
d gene (50.2A) indicate that the B10.GAA37 mouse strain has a multi-locus D (D
w16) region distinct from the five-gene organization observed in the D
d and D
q regions. To isolate the D
w16 region class I genes, a genomic B10. GAA37- EMBL3 library was generated and screened with probes that preferentially hybridize to K and D region class I genes. Hybridization analyses of the isolated clones with L
d derived oligonucleotide probes suggested that one of the clones contained the L
w16 gene, whereas several other clones contained the L
w16 gene. The sequence of the D
w16 gene is most similar to that of the D
p gene, particularly in the 3 half. Furthermore, the L
w16 gene is quite similar in the 5 half and virtually identical in the 3 half to the L
d gene, indicating that L
w16, but not D
w16, is a member of the L
d gene family. Collectively, these data suggest that, through a D region recombination event, the novel D
w16 region may have been assembled from primordial counterparts of the D
p and L
d genes.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M60774-M60776, and M62759. |
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Keywords: | |
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