Analysis of human metaphase chromosomes using antibodies to double-stranded and single-stranded DNA: staining patterns are related to DNA conformation |
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| Authors: | R Mezzanotte D Peretti M G Ennas R Vanni A T Sumner |
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| Institution: | Istituto di Biologia Generale, Università di Cagliari, Italy. |
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| Abstract: | Fresh and 6-day-old fixed chromosome spreads, both untreated and treated with various banding techniques and nucleases, were stained using monoclonal antibodies to double-stranded and single-stranded DNA. DNA in fixed chromosome preparations became progressively denatured with ageing. The staining pattern of untreated chromosomes with anti-double-stranded DNA antibodies (which resembles G-banding) was determined by the conformation of the chromosomal DNA. |
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