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Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from <Emphasis Type="Italic">Bifidobacterium longum</Emphasis> |
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| Authors: | Taeyeon Kwon Cheong Tae Kim Jong-Hoon Lee |
| Institution: | (1) Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, South Korea;(2) Nong Shim Co., LTD., Kunpo, 435-713, South Korea |
| Abstract: | A novel transglycosylation reaction from sucrose to l-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable l-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-α-d-glucopyranosyl-l-ascorbic acid by LC-MS/MS analysis. |
| Keywords: | l-Ascorbic acid" target="_blank">l-Ascorbic acid Bifidobacterium longum 2-O-α d-Glucopyranosyl-l-ascorbic acid" target="_blank">-d-Glucopyranosyl-l-ascorbic acid Sucrose phosphorylase Transglucosylation |
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