A human islet cell culture system for high-throughput screening |
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Authors: | Walpita Deepika Hasaka Thomas Spoonamore James Vetere Amedeo Takane Karen K Fomina-Yadlin Dina Fiaschi-Taesch Nathalie Shamji Alykhan Clemons Paul A Stewart Andrew F Schreiber Stuart L Wagner Bridget K |
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Institution: | Chemical Biology Program, Broad Institute, Cambridge, MA 02142, USA. |
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Abstract: | A small-molecule inducer of beta-cell proliferation in human islets represents a potential regeneration strategy for treating type 1 diabetes. However, the lack of suitable human beta cell lines makes such a discovery a challenge. Here, we adapted an islet cell culture system to high-throughput screening to identify such small molecules. We prepared microtiter plates containing extracellular matrix from a human bladder carcinoma cell line. Dissociated human islets were seeded onto these plates, cultured for up to 7 days, and assessed for proliferation by simultaneous Ki67 and C-peptide immunofluorescence. Importantly, this environment preserved beta-cell physiological function, as measured by glucose-stimulated insulin secretion. Adenoviral overexpression of cdk-6 and cyclin D(1), known inducers of human beta cell proliferation, was used as a positive control in our assay. This induction was inhibited by cotreatment with rapamycin, an immunosuppressant often used in islet transplantation. We then performed a pilot screen of 1280 compounds, observing some phenotypic effects on cells. This high-throughput human islet cell culture method can be used to assess various aspects of beta-cell biology on a relatively large number of compounds. |
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