IL-4/Stat6 activities correlate with apoptosis and metastasis in colon cancer cells |
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Authors: | Li Ben Hui Yang Xian Zi Li Pin Dong Yuan Qin Liu Xiao Hong Yuan Jia Zhang Wen Jie |
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Institution: | a Department of Medical Oncology, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan, Hubei 430071, China b Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei 430030, China c Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China d Department of Medical Oncology, Taihe Hospital, Yunyang Medical College, Shiyan, Hubei 442000, China |
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Abstract: | IL-4-induced Stat6 signaling is active in a variety of cell types and plays a role in cell proliferation/growth and resistance to apoptosis. Using EMSA, we identified differential IL-4/Stat6 activities in colorectal cancer cell lines, HT-29 being active Stat6high phenotype and Caco-2 being defective Stat6null phenotype, respectively. Active Stat6high HT-29 cells exhibited resistance to apoptosis by flowcytometry and aggressive metastasis by Transwell assay compared with defective Stat6null Caco-2 cells. Comparing one another using RT-PCR, Stat6high HT-29 cells expressed more mRNA of anti-apoptotic and pro-metastatic genes Survivin, MDM2, and TMPRSS4, while Stat6null Caco-2 cells expressed more mRNA of pro-apoptotic and anti-metastatic genes BAX, CAV1, and P53, respectively. This is the first study describing correlations of IL-4/Stat6 activities with apoptosis and metastasis in colon cancer. These findings, together with the observation of constitutive Stat6 activation in many human malignancies, suggest that Stat6 activities could be a biomarker for cancer cell’s invasive/metastatic capability. |
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Keywords: | Apoptosis Caco-2 Colorectal cancer EMSA HT-29 IL-4 Metastasis Stat6 |
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