Inhibitory effect of obestatin on glucose-induced insulin secretion in rats |
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Authors: | Ren An-Jing Guo Zhi-Fu Wang Yang-Kai Wang Li-Gang Wang Wei-Zhong Lin Li Zheng Xing Yuan Wen-Jun |
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Affiliation: | a The Department of Physiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China b The Department of Cardiovascular Diseases, Changhai Hospital, Second Military Medical University, Shanghai 200433, China c School of Basic Medical Science, Ning-Xia Medical College, Yinchuan 750004, China |
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Abstract: | Obestatin is a bioactive peptide encoded by the same gene that encodes ghrelin. Our aim was to investigate the effect of obestatin on insulin secretion. We evaluated the effects of obestatin on insulin secretion from rat islet cells which had been incubated overnight in the presence of 8.3, 11.1, and 22.2 mmol/l of glucose. In vivo, the serum levels of glucose and insulin were measured 0, 1, 5, 10, 20, 40, and 60 min after the intravenous administration of saline or glucose (1 g/kg), with or without obestatin, and the area under the 60 min curve of insulin concentration (AUCinsulin) was calculated. Obestatin (0.01-100 nmol/l) inhibited insulin secretion from rat islets in a dose-dependent fashion. In vivo, when administered intravenously to rats together with glucose, obestatin (10, 50, and 250 nmol/kg) inhibited both the rapid 1-min insulin response and the AUCinsulin in a dose-dependent fashion. Our data demonstrate that under glucose-stimulated conditions, exogenous obestatin acts as a potent inhibitor of insulin secretion in anaesthetized rats in vivo as well as in cultured islets in vitro. |
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Keywords: | Obestatin Ghrelin Glucose Insulin |
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