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Cellular prion protein in mammary gland and milk fractions of domestic ruminants |
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| Authors: | Didier A Gebert R Dietrich R Schweiger M Gareis M Märtlbauer E Amselgruber W M |
| Affiliation: | a Chair for Hygiene and Technology of Milk, Ludwig Maximilians University, Schoenleutnerstraße 8, 85764 Oberschleissheim, Germany b Institute for Anatomy and Physiology of Domestic Animals, University of Hohenheim, 70599 Stuttgart, Germany c Institute for Microbiology and Toxicology, Federal Research Institute for Nutrition and Food (MRI), 95326 Kulmbach, Germany |
| Abstract: | The present study shows that PrPc is expressed in the mammary gland and milk fractions of domestic ruminants in a species-specific manner. By applying immunohistochemistry, Western blot and ELISA, clear expression differences between bovine, ovine and caprine mammary gland, skimmed milk, acid whey and cream could be demonstrated, the highest relative PrPc levels being associated with the cream fraction. In the bovine gland PrPc was preferentially detectable at the basolateral surface of mammary gland epithelial cells, whereas in ovine and caprine samples the prion protein was more homogeneously distributed. Moreover, in ovine and caprine bovine mammary gland epithelial cells, apocrine secretory vesicles were strongly stained. Ovine and caprine milk proved to contain PrPc in all fractions with an additional truncated form at 12 kDa in Western blot. This truncated isoform is the predominate one in caprine acid whey. These results support the hypothesis that the apocrine secretion mode of milk fat globules is a major way of PrPc transport into the milk. |
| Keywords: | Apocrine secretion Cellular prion protein Mammary gland Milk |
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