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Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2 |
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| Authors: | Chang Chih-Hung Fang Hsu-Wei Ho Yi-Ching Huang Huei-Ting |
| Institution: | a Division of Orthopaedics, Department of Surgery, Far Eastern Memorial Hospital, Taipei, Taiwan b Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Tao-Yuan, Taiwan c Department of Chemical Engineering & Biotechnology, Institute of Biotechnology, National Taipei University of Technology, 1, Section 3, Chung-Hsiao E. Road, Mailbox 2521, Taipei 106, Taiwan |
| Abstract: | It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE2. In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis. |
| Keywords: | Phagocytosis Chondrocyte Wear particles UHMWPE Partial joint arthroplasty |
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