Immunologic reactions of rabbit anti-Mycoplasma arthritidis serum with in vitro cultivated rat synovial cells |
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Authors: | Charles Edward Thirkill Harold G Muchmore Richard M Hyde L V Scott |
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Institution: | (1) Department of Ophthalmology and Visual Science, Yale University School of Medicine, 06510 New Haven, Connecticut;(2) Department of Microbiology and Immunology, University of Oklahoma Health Science Center, 73190 Oklahoma City, Oklahoma |
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Abstract: | Summary The pathogenesis of the intra-articular, arthritic-inflammatory reaction caused byMycoplasma arthritidis in susceptible rats and mice is poorly understood. To investigate this problem, synovial cells from normal Sprague-Dawley
rats were cultured and studied in vitro. These cells continued to produce hyaluronic acid as measured by viscosity and chemical
assays. Normal synovial cells were treated with rabbit serum specimens taken before and after immunization withM. arthritidis. Cytotoxicity assays indicated that the cells were killed in the presence of rabbit anti-M. arthritidis serum but not with preimmunization serum specimens. The anti-M. arthritidis serum was not cytotoxic to monolayer cultures of HEp-2, Vero, or L-cells. Antiserum produced in response toM. fermentans, M. hominis, andM. pulmonis did not produce a cytotoxic effect on the cultured synovial cells. From immunofluorescence studies it was demonstrated that
the interactions occurred between the rabbit anti-M. arthritidis serum and synovial cell surface antigens. Extreme precautions were taken to prevent mycoplasmal contamination of rats and
the synovial cells in culture. These observations would appear to support previous reports implicating mycoplasmas as biological
triggering mechanisms of autoimmune reactions.
This research was supported in part by funds from the National Science Foundation, Grant DPP72-05787, and the U.S. Veterans
Administration. |
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Keywords: | primary rat synovial cell cultures viscosity assay arthritogenic mycoplasmas cytotoxicity immunofluorescence antibody binding |
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