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High cell density perfusion culture of hybridoma cells recycling high molecular weight components |
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| Authors: | Yoshiharu Takazawa Michiyuki Tokashiki Kimihiko Hamamoto Hiroki Murakami |
| Affiliation: | (1) Biotechnology Research Laboratory, Teijin Limited, 191 Hino, Japan;(2) Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, 46-09, Fukuoka, 812, Japan;(3) Biotechnology Research Laboratory, Teijin Tokyo Research Center, 4-3-2 Asahigaoka, 191 Hino, Tokyo, Japan |
| Abstract: | We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration. |
| Keywords: | high-density hybridoma monoclonal antibody perfusion culture serum-free suspension culture |
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