Effects of diabetes and insulin on ketone bodies metabolism in heart |
| |
Authors: | A M N Sultan |
| |
Institution: | (1) Department of Medical Sciences Umm Al-Qura University Makkah, Saudi Arabia |
| |
Abstract: | This work investigates the effect of alloxan-induced short-term diabetes (24 h) on D-3-hydroxybutyrate metabolism at physiological
and non-physiological concentrations of the ketone body in the isolated non-working perfused rat heart. Also the effect of
insulin (2 mU.ml−1) on D-3-hydroxybutyrate metabolism was investigated in hearts from normal and diabetic rats. The rates of D-3-hydroxybutyrate
utilization and oxidation and of acetoacetate production were proportional to D-3-hydroxybutyrate concentration. The utilization
of D-3-hydroxybutyrate showed saturation kinetics in hearts from normal and diabetic rats, in the presence and absence of
insulin. Acute short-term diabetes augmented D-3-hydroxybutyrate utilization and oxidation at 1.25 and 2.5 mM DL-3-HB, with
no significant effect at higher concentrations, but increased acetoacetate production at all investigated concentrations.
In hearts from normal rats, insulin enhanced D-3-hydroxybutyrate utilization and oxidation at 2.5, 5, and 10 mM DL-3-HB, but
no effect was observed at the lowest (1.25 mM) and highest (16 mM) DL-3-HB concentrations. Insulin had no effect on D-3-hydroxybutyrate
metabolism in hearts from diabetic rats. No significant effect of insulin on the rate of acetoacetate production in normal
and diabetic states was observed. |
| |
Keywords: | diabetes insulin hypoinsulinemia ketone bodies D-3-hydroxybutyrate acetoacetate cardiac metabolism |
本文献已被 SpringerLink 等数据库收录! |
|