Introduction of the mercury transposon Tn501 into Rhizobium japonicum strains 31 and 110 |
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Authors: | G.S. Bullerjahn Rolf H. Benzinger |
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Affiliation: | Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, VA 22901, U.S.A. |
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Abstract: | Abstract Transposon Tn 501 , which encodes resistance to mercuric ions, was introduced into Rhizobium japonicum 110 and 31 by conjugal transfer. The transposon donor plasmid (pMD100) was able to mobilize into R. japonicum , but could not be maintained. Hg2+-resistant colonies were recovered at a frequency of 1.9 × 10−8/recipient for strain 110, and 1.7 × 10−7/recipient for strain 31. Presence of Tn 501 in Hg-resistant isolates was verified by Southern analysis and demonstrating transposition of Hg resistance. Transposon mutagenesis has been used to generate auxotrophic mutations at low frequency. |
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Keywords: | Tn501 transposon insertion Rhizobium |
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