Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.) |
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Authors: | D Ernst D Oesterhelt W Schäfer |
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Institution: | (1) Max-Planck-Institut für Biocheme, D-8033 Martinsried, Federal Republic of Germany |
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Abstract: | Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growth (embryonal: isopentenyladenosine, 4 ng g-1 fresh weight FW]; isopentenyladenine, 1.4 ng g-1 FW; zeatin, 3.6 ng g-1 FW; proembryonal: isopentenyladenosine, 58.3 ng g-1 FW; isopentenyladenine, 7.9 ng g-1 FW; zeatin 11.1 ng g-1 FW). The proembryonic culture medium but not the embryonic culture medium contained isopentenyladenosine up to 28 pg ml-1 during logarithmic growth. No correlation between different embryonic stages and the endogenous cytokinin level was obvious.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- FW
fresh weight
- GC-MS
gas chromatography-mass spectrometry
- HPLC
high-performance liquid chromatography
- RIA
radioimmunoassay
- SIM
single-ion monitoring |
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Keywords: | Cell culture Cytokinin and embryogenesis Embryonic/proembryonic development Pimpinella |
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