Effect of Light and NaCI Salinity on the Growth of Callus Cultures of Ipomoea pes-caprae (L.) R. Brown and Ipomoea batatas (L.) Lam |
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Authors: | OCHOA-ALEJO N; LOPEZ-GUTIERREZ F |
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Institution: | 1 Centro de Investigación y de Estudios Avanzados del IPN-Unidad Irapuato Apartado Postal 629, 36500-Irapuato, Gto., México
2 Centro de Investigaciones Biológicas de Baja California Sur, A.C. Apartado Postal 128, 23060-La Paz, B.C.S., México |
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Abstract: | Callus cultures of Ipomoea pes-caprae and I. batatas were establishedon MS medium containing 105 M 2,4-D and 108 Mbenzyladenine. Ipomoea pes-caprae calli exhibited green pigmentationand grew better in the light than in darkness. Callus tissuesof I. batatas showed a pale-yellow colouration and they grewat the same rate in light as in dark conditions. I. pes-capraeand I. batatas callus cultures were sensitive to the presenceof 60 mM NaCl in the culture medium, the growth of the formerbeing more sensitive in light than in darkness. The significanceof the responses of I. pes-caprae callus cultures in relationto the mechanism of salt tolerance is discussed. Ipomoea batatas, Ipomoea pes-caprae, sweet potato, railroad vine, callus cultures, salinity, light |
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