Development of low-cost microfluidic systems for lab-on-a-chip biosensor applications |
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Authors: | Adisorn Tuantranont Tahom Lomas Thitima Maturos Anurat Wisitsora-at Panote Thavarungkul Proespichaya Kanatharana Warakorn Limbut Suchera Loyprasert |
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Institution: | (1) Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center, 112 Thailand Science Park, Pahol Yothin Rd., Klong Laung, 12120 Pathumthani, Thailand;(2) Faculty of Science, Prince of Songkla University, Hat Yai, 90112 Songkhla, Thailand |
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Abstract: | In this work, we develop low-cost microfluidic systems based on polydimethylsiloxane (PDMS) for lab-on-a-chip applications.
PDMS microfluidic structures have been fabricated by micromolding, PDMS casting, and plasma bonding processes. The micromolding
technique is used to fabricate PDMS slabs with micro-sized grooves, and the complete microchannel is formed by bonding PDMS
slab with glass or PDMS substrate. The molding procedure using SU-8 photoresist patterning on silicon wafer, PDMS microchannel
fabrication, and PDMS surface treatment using oxygen plasma and TiO2 coating, are discussed. The various parameters for oxygen plasma treatment including RF power and treatment time are studied
in order to obtain conditions for good bonding with the glass substrate. The best condition for plasma treatment is found
to be the low RF power (8 W) with 5 min treatment time. In addition, TiO2 coating with oxygen plasma treatment has been applied to make PDMS surface more hydrophilic to improve aqueous solution compatilbility.
The microfluidic channels for various applications, including sample injection cross channel, micropump channel, T and Y sample
mixers, PCR thermocyling chamber and channel, capillary electrophoresis flow channel, and conductimetric systems have been
fabricated. Finally, a typical application of the PDMS chip in a flow injection conductimetric system for sodium chloride
detection has been demonstrated. |
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Keywords: | Polydimethylsiloxane micromolding microfluidie lab-on-a-chip |
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