A batchwise purification procedure of neurofilament proteins |
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| Authors: | S Tokutake S B Hutchison J S Pachter R K Liem |
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| Affiliation: | Department of Pharmacology, New York University School of Medicine, New York, New York 10016 USA |
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| Abstract: | A rapid batchwise purification procedure for neurofilament proteins from bovine spinal cord is described. A crude filament fraction can be obtained by treating the tissue with Triton X-100, followed by centrifugation through sucrose. From this crude filament fraction the protein is processed through a batch purification procedure using hydroxyapatite in 8 M urea. With this procedure, approximately 0.5 g of purified neurofilament protein is obtained from a single bovine spinal cord in less than 3 days. |
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| Keywords: | To whom correspondence should be addressed. |
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