Thesec andprl genes ofEscherichia coli |
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Authors: | Kristina L. Bieker Gregory J. Phillips Thomas J. Silhavy |
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Affiliation: | (1) Department of Biology, Princeton University, 08544 Princeton, New Jersey |
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Abstract: | Two general approaches have been used to define genetically the genes that encode components of the cellular protein export machinery. One of these strategies identifies mutations that confer a conditional-lethal, pleiotropic export defect (sec,secretion). The other identifies dominant suppressors of signal sequence mutations (prl,proteinlocalization). Subsequent characterization reveals that in at least three cases,prlA/secY,prlD/secA, andprlG/secE, both types of mutations are found within the same structural gene. This convergence is satisfying and provides compelling evidence for direct involvement of these gene products in the export process. |
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Keywords: | Protein secretion signal sequence suppressors hybrid proteins gene fusions |
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