低分子量硫酸葡聚糖对小鼠造血干细胞动员作用的研究

给小鼠静脉注射低分子量（＜１０￣４ｕ）硫酸葡聚糖（ＤＳ）１５ｍｇ／ｋｇ后外周血中白细胞、单个核细胞（ｍｏｎｏｎｕｃｌｅａｒｃｅｋ，ＭＮＣ）、ＣＦＵ－ＧＭ、ＢＦＵ－Ｅ和ＣＦＵ－Ｍｉｘ产率等指标出现时相性变化。给药后１ｈ开始升高，２ｈ达到高峰、分别为药前值的２．２、２．６、３．８、４．４和３．０倍，７ｈ时趋向正常。给药后２ｈ上述各类细胞在外周血中的含量随着ＤＳ的剂量增加而增加。白细胞、ＭＮＣ计数在ＤＳ１８０ｍｇ／ｋｇ时达到峰值，均为对照组的４倍。２４０ｍｇ／ｋ８时未见明显增加。不同剂量ＤＳ对各系祖细胞均有不同程度的动员作用，ＤＳ剂量１５－３０ｍｇ／ｋｇ效果最好，每升血中ＣＦＵ－ＧＭ、ＢＦＵ－Ｅ、ＣＦＵ－Ｍｉｘ的数量分别相当于对照组的５．０、１１．９和８．８倍。其峰值出现时间与白细胞、ＭＮＣ不同，表明ＤＳ对不同类型细胞的作用机制也不尽一致。经口给小鼠投以ＤＳ２４０和４８ｏｍｇ／ｋｇ后，未见外周血中白细胞、ＭＮＣ计数有显著性升高，提示对造血干细胞没有动员作用。

THE ROLE OF DEXTRAN SULFATE WITH LOW MOLECULARWEIGHT IN MOBILIZATION OF THE STEM CELLS IN MICE

Healthy NIH mice weiging 2 26g were used in this study.One hour aner injection ofdextran sulfate(DS),the numbers of WBC,MNC,CFU-GM,BFU-E and CFU-Mix in theperipheral blood increased and achieved peak values at 2 hours after injiction,which were 2.2,2.6,3.8,4.4 and 3.0 folds of the preinjection values respectively.They returned to noimal levels at7 hours after injection. The numbers of WBC,MNC,CFU-GM,BFU-E and CFU-Mix inmouse blood began to increase after injection of DS 7.5mg/kg and WBC,MNC acieved peakvalues at 180mg/kg(P<0.001),DS 15 30mg kg had the best efficacy for mobilization ofCFU-GM,BFU-E and CFU-Mix,causing 5.0,11.9 and 8.8 fold-increase(per liter of bood,P<0.001).No increase in WBC and MNC in blood was found after oral administration of DS240 and 480mg/kg.