| 基因修饰的小鼠肥大细胞瘤细胞的酶细胞化学变化 |
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| 引用本文： | 刘晓丹 郭宁. 基因修饰的小鼠肥大细胞瘤细胞的酶细胞化学变化[J]. 中国应用生理学杂志, 1996, 12(3): 230-232 |
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| 作者姓名： | 刘晓丹 郭宁 |
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| 作者单位： | 军事医学科学院基础医学研究所，解放军第三０七医院 |
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| 摘 要： | 应用酶细胞化学方法对基因转导前后的Ｐ８１５小鼠肥大细胞瘤细胞进行酸性磷酸酶、非特异性酯酶及多糖的定位。研究结果显示，空载体转导的Ｐ８１５／ｎｅｏ细胞与其亲本细胞相比无显著改变，而ＩＬ２基因修饰的Ｐ８１５细胞的酸性磷酸酶及多糖反应明显增强，可能提示细胞内某些细胞器的形成以及细胞内多糖类合成功能的增强。
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| 关 键 词： | 逆转录 病毒载体 基因转移 酶细胞化学 |
ENZYMIC CYTOCHEMICAL CHANGES OF GENE MODIFIED MOUSE MASTOCYTOMA CELLS |
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| Liu Xiaodan,Guo Ning,Li Xiusen,Li Fumeng,Liu Shujun,Mao Ning. ENZYMIC CYTOCHEMICAL CHANGES OF GENE MODIFIED MOUSE MASTOCYTOMA CELLS[J]. Chinese journal of applied physiology, 1996, 12(3): 230-232 |
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| Authors: | Liu Xiaodan Guo Ning Li Xiusen Li Fumeng Liu Shujun Mao Ning |
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| Abstract: | Acid phosphatase, acid naphthyl esterase, and periodic acid Schiff(PAS)reaction in P815 mastocytoma cells were detected by using cytochemical methods before and after gene transduction. The results showed that P815/neo cells transduced with the retroviral vector containing only neo gene had no remarkable changes of the enzymic activities compared with the parental cells. However acid phosphatase and PAS reaction of IL 2 gene modified P815 cells were markedly increased. The findings may suggest the formation of some organelles and active function of producing polysaccharide in IL 2 gene modified P815 cells. |
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| Keywords: | retroviral vector gene transfer enzymic cytochemistry |
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